#Western blot protein ladder bio rad plus#
#Western blot protein ladder bio rad free#

Precision Plus Protein WesternC standards - these standards were designed for western blotting applications.

Precision Plus Protein Dual Xtra standards - these prestained standards have additional 2 and 5 kD bands to enable molecular mass estimation below 10 kD.

The ability to visualize these standards makes them ideal for monitoring protein separation during gel electrophoresis and monitoring transfer onto blotting membranes.

Precision Plus Protein prestained standards (All Blue, Dual Color, and Kaleidoscope™) - include a proprietary staining technology that provides batch-to-batch molecular mass consistency and reproducible migration.

Bio-Rad offers both recombinant and natural prestained standards. The prestained markers remain visible on the membrane after transfer, which makes them useful for monitoring transfer efficiency and the general location of antigens in repetitive screening assays (Tsang et al. The proteins are labeled with fluorescent dyes, so can be used in fluorescence detection applications. The ability to visualize prestained standards during electrophoresis makes them ideal for monitoring protein separation during SDS-PAGE. Overview of the StrepTactin detection system It is the high-affinity binding of the Strep-tag sequence to StrepTactin that allows convenient and simultaneous detection of both proteins and standards on western blots (see figure below) using either colorimetric or chemiluminescence methods.

These standards contain an affinity StrepTactin, a genetically modified form of streptavidin.

#Western blot protein ladder bio rad free#

Additionally, these standards are compatible with Laemmli and neutral pH buffer systems and are an excellent choice for use with the stain free technology (since they do not contain dye that can interfere with stain-free detection). These standards offer absolute molecular weight accuracy confirmed by mass spectrometry. Precision Plus Protein unstained standards - include three high-intensity reference bands (25, 50, and 75 kD).Therefore, unstained standards or standards with affinity tags for blot detection deliver high molecular weight recommended for the most accurate molecular weight determinations for gels or blots. Unstained standards contain only purified proteins, so they do not exhibit the variability in molecular weight sometimes observed with prestained standards. Precision Plus Protein family of protein standards. These standards contain highly purified recombinant proteins with molecular masses of 10–250 kD (or 2–250 kD for the Dual Xtra standards). Bio-Rad's recombinant standards are the Precision Plus Protein™ standards family of products and are available in stained or unstained formats (see figure below). Recombinant standards are engineered to display specific attributes such as evenly spaced molecular weights or affinity tags for easy detection on western blots. * For colorimetric or chemiluminescence blot detection, StrepTactin-HRP or -AP conjugate is also needed. Prestained multicolored fluorescent bands, multicolored band pattern Kaleidoscope Prestained Standards (Natural) Precision Plus Protein™ All Blue Standards Prestained multicolored fluorescent bands, 5-color band pattern Precision Plus Protein™ Kaleidoscope™ Standards Prestained multicolored fluorescent bands, 2-color band pattern, extended MW range Precision Plus Protein Dual Xtra Standards Prestained multicolored fluorescent bands, 2-color band pattern Precision Plus Protein Dual Color Standards Prestained multicolored fluorescent band with integrated Strep-tag for chemiluminescence visualization Precision Plus Protein™ WesternC™ Standards Integrated Strep-tag for chemiluminescence visualization* Precision Plus Protein™ Unstained Standards Protein blended to yield uniform band intensities upon staining

Protein standards for western blotting applications selection guide. Recombinant Precision Plus Protein Standards **Precision Plus Protein Standard Plugs are designed specifically for second dimension SDS-PAGE gels in 2-D experiments.īio-Rad's protein standards for western blotting applications. See bulletin 5723 for details on using precision Plus Protein WesternC standards for fluorescent multiplexing. Precision Plus Protein prestained standards contain dyes with fluorescent properties. * Not to be confused with fluorescent total blot stains. Applications of Bio-Rad's protein standards.